Postdoctoral Research Scholar
North Carolina State University at Raleigh Raleigh, NC
We are a dynamic research team focused on elucidating the molecular mechanisms of plant-virus-vector interactions. Our mission is to develop new foundational knowledge of plant viruses and to translate this information into new control strategies and biotechnology tools for crop improvement.
Grapevine red blotch disease has emerged as a major threat to winegrape production throughout North America in recent decades. We aim to develop tools and protocols for grapevine red blotch virus (GRBV) detection in insects and plants. We will integrate these new technologies with established methods to better understand GRBV localization in the insect vector throughout circulative transmission and make strides in understanding GRBV latency in grapevine and experimental plant hosts. The detection tools developed will also have applications for red blotch diagnostics.
Duties and Responsibilities:
Grapevine red blotch disease has emerged as a major threat to winegrape production throughout North America in recent decades. We aim to develop tools and protocols for grapevine red blotch virus (GRBV) detection in insects and plants. We will integrate these new technologies with established methods to better understand GRBV localization in the insect vector throughout circulative transmission and make strides in understanding GRBV latency in grapevine and experimental plant hosts. The detection tools developed will also have applications for red blotch diagnostics.
Duties and Responsibilities:
- The postdoctoral research scholar will express GRBV proteins in prokaryotic and eukaryotic (insect and plant) cells and purify proteins for antibody production. (50%)
- Characterize GRBV dissemination and tissue tropism in insect vectors with in situ methods. (50%)
- Molecular analysis of viral genomes, gene expression, and gene function.
- Primer design, DNA/RNA handling, protein expression, Western blotting.
- Develop and test standardized procedures for determining vector competence on candidate vectors.
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